Document Type

Article

Publication Date

11-22-2025

Abstract

Accurate fish species identification is critical to prevent mislabeling and fraud in the seafood industry. We present a handheld multi-mode point spectroscopy system that combines fluorescence (365 and 395 nm excitation) and reflectance measurements in the visible to near-infrared (∼350–900 nm) and short-wave infrared (∼900–1700 nm) regions for rapid, non-destructive classification of fish fillets. Tissue spectra were acquired at 25 positions on 68 fillets from 11 species, in both frozen and thawed states. Feature-level fusion across all four modes enabled higher classification accuracy than any single mode alone. A global machine-learning model classified all species with 85 ± 2.8 %, while specialized dispute models for commonly misclassified species improved performance to 90 % ± 6.1 %. Individual models for thawed and frozen fillets achieved 90 ± 6.0 % and 90 ± 5.4 %, respectively, with dispute models in the thawed dataset increasing accuracy to 93 ± 4.3 %. These results demonstrate that portable multi-mode spectroscopy, combined with machine learning, provides a fast and reliable tool for on-site fish species identification.

Comments

This article was originally published in Applied Food Research, volume 5, issue 2, in 2025. https://doi.org/10.1016/j.afres.2025.101536

Peer Reviewed

1

Copyright

The authors

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

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