Microplate Immunocapture Coupled with the 3M Molecular Detection System and Selective Plating for the Rapid Detection of Salmonella infantis in Dry Dog Food and Treats

Authors

Danielle K. Rosen, Chapman University
Miguel Gallardo, Chapman University
McClain Vail, Chapman University
Rosalee S. Hellberg, Chapman UniversityFollow

Document Type

Article

Publication Date

2-28-2020

Abstract

The objective of this study was to use microplate immunocapture (IC) to reduce the enrichment time required for detection of Salmonella in pet food with the 3 M Molecular Detection System (MDS) or selective plating on XLD. Dog food and pig ear treats were inoculated with Salmonella Infantis at concentrations of 10⁰–10⁴ CFU/25 g, followed by a 3-h enrichment, then microplate IC and 3 M MDS or microplate IC and selective plating on XLD. Another set of samples underwent a traditional 24-h enrichment followed by 3 M MDS or selective plating. Based on the results of three independent trials, microplate IC followed by selective plating enabled detection of Salmonella in 100% of dog food and treat samples tested, including at levels as low as 10⁰ CFU/25 g. Microplate IC coupled with 3 M MDS enabled detection of Salmonella in dog food and treat samples down to levels of 10⁰ CFU/25 g, with an overall detection rate of 92%. These results indicate high potential for microplate IC to be used in place of the traditional 24-h enrichment step, enabling detection of Salmonella in complex matrices when coupled with 3 M MDS or selective plating.

Comments

NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Microbiological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Microbiological Methods, volume 172, in 2020. https://doi.org/

The Creative Commons license below applies only to this version of the article.

Recommended Citation

Rosen, D.K., Gallardo, M., Vail, M., Hellberg, R.S. (2020). Microplate immunocapture coupled with the 3M molecular detection system and selective plating for the rapid detection of Salmonella infantis in dry dog food and treats. Journal of Microbiological Methods, 172, 105881. https://doi.org/10.1016/j.mimet.2020.105881

Peer Reviewed

1

Copyright

Elsevier

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.