Rings of Power: Controlling SOD Mimic Activity by the Addition of Pyridine Rings within the Pyridinophane Scaffold

Authors

• Katherine J. Smith, Texas Christian University
• Timothy M. Schwartz, Texas Christian University
• David M. Freire, Texas Christian University
• Cameron Bowers, Texas Christian University
• Sarah K. Dunn, Texas Christian University
• Jackson F. Bonnell, Texas Christian University
• Magy A. Mekhail, Chapman UniversityFollow
• Giridhar Akkaraju, Texas Christian University
• Kayla N. Green, Texas Christian University

Document Type

Article

Publication Date

12-3-2024

Abstract

Superoxide dismutase enzymes are a major defense against superoxide, which is a potent reactive oxygen species. Misregulation of reactive oxygen species and subsequent neuronal damage are etiological hallmarks of neurodegenerative disease. Macrocyclic small molecules have offered inroads toward functional SOD1 mimics. Herein, we report a series of five tetra-aza macrocyclic ^RPy₂N₂ ligands, varied by 4-position substitution of the pyridine ring with both electron-donating (R = OMe) and withdrawing groups (R = Cl, I, and CF₃) to offer the first comparison to well-studied ^RPyN₃ congeners and other mimics in the literature. New ligands have been characterized by NMR, mass spectrometry, elemental analysis, and potentiometric titrations (pK_a). Cyclic voltammetry and X-ray diffraction analysis of the copper(II) complexes (Cu^II(^RPy₂N₂)²⁺) demonstrate how pyridine substitution impacts the metal center. This data, and evaluation of the log β_Cu(II) and log β_Cu(I) within the series, indicates significant improvement to the binding affinity for Cu(I) without sacrifice of Cu(II) binding. The Cu^II(^RPy₂N₂)²⁺ series yield the highest k_cat for any Cu(II)-based small molecule functional SOD1 mimic (k_cat = 45.36 M^–1 s^–1). Furthermore, the Cu^II(^OMePy₂N₂)²⁺ and Cu^II(^CF₃Py₂N₂)²⁺ complexes were studied in FRDA cells to determine cell toxicity as a first step toward the application of these mimics as therapeutics for neurological disease.

Comments

This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Inorganic Chemistry, volume 63, issue 50, in 2024 following peer review. This article may not exactly replicate the final published version. The definitive publisher-authenticated version is available online at https://doi.org/10.1021/acs.inorgchem.4c02776.

Recommended Citation

Smith, K. J.; Schwartz, T.; Freire, D.M.; Bowers, C.; Dunn, S.; Bonnell, J.; Mekhail, M. A.; Akkaraju, G; Green, K. N. Rings of Power: Controlling SOD Mimic Activity by the Addition of Pyridine Rings within the Pyridinophane Scaffold. Inorganic Chemistry 2024, 63 (50) 23544–23553. https://doi.org/10.1021/acs.inorgchem.4c02776

Copyright

American Chemical Society