"Abstract 2853 PRMT7 Negatively Regulates the Expression p53 in Respons" by Molly Niswender, Lorenzo Pessi et al.
 

Document Type

Article

Publication Date

6-13-2025

Abstract

Protein Arginine Methyltransferase 7 (PRMT7) is the only member of the protein arginine methyltransferase protein family that monomethylates its protein substrates. PRMT7 is found in both the nucleus and cytoplasm of breast cells and is believed to play a robust role in the tumorigenesis and metastasis of breast cancer. The goal of this project is to uncover possible pathways for PRMT7 to promote cancer progression. A preliminary antibody array was performed to determine the regulation of known cancer-related proteins by PRMT7. An early-stage human breast cancer cell line, MCF-7, was transfected with plasmid pCDH1-hPRMT7-GFP to over-express PRMT7. Qualitative and quantitative analysis of detectable expression changes compared to sham transfection revealed a decrease in p53 expression, a protein not previously associated with PRMT7. Upon DNA damage, p53 can induce the expression of genes like p21, which inhibit cyclin-dependent kinases (CDKs) and halt the cell cycle. This pause allows the cell time to repair the DNA damage before proceeding with division. Our current research aims at identifying the mechanisms of action by which PRMT7 regulates p53. For example, PRMT7 may monomethylate proteins directly implicated in the expression or activity of p53. Successful completion of this project will provide novel insights into how PRMT7 contributes to breast cancer progression.

Comments

Originally published in Journal of Biological Chemistry, volume 301, issue 5, supplement, in 2025. https://doi.org/10.1016/j.jbc.2025.108752

Copyright

The American Society for Biochemistry and Molecular Biology, Inc.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.