Microcin C is a ribosome-synthesized heptapeptide that contains a modified adenosine monophosphate covalently attached to the C-terminal aspartate. Microcin C is a potent inhibitor of bacterial cell growth. Based on the in vivo kinetics of inhibition of macromolecular synthesis, Microcin C targets translation, through a mechanism that remained undefined. Here, we show that Microcin C is a subject of specific degradation inside the sensitive cell. The product of degradation, a modified aspartyl-adenylate containing an N-acylphosphoramidate linkage, strongly inhibits translation by blocking the function of aspartyl-tRNA synthetase.
Metlitskaya, A., Kazakov, T., Kommer, A., Pavlova, O., Prætorius-Ibba, M., Ibba, M. Krasheninnikov, I., Kolb, V., Khmel, I. and Severinov, K. (2006) Aspartyl-tRNA synthetase is the target of peptidenucleotide antibiotic Microcin C. J. Biol. Chem 281, 18033 - 18042. https://doi.org/10.1074/jbc.M513174200
American Society for Biochemistry and Molecular Biology
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