Document Type
Article
Publication Date
3-30-2006
Abstract
Microcin C is a ribosome-synthesized heptapeptide that contains a modified adenosine monophosphate covalently attached to the C-terminal aspartate. Microcin C is a potent inhibitor of bacterial cell growth. Based on the in vivo kinetics of inhibition of macromolecular synthesis, Microcin C targets translation, through a mechanism that remained undefined. Here, we show that Microcin C is a subject of specific degradation inside the sensitive cell. The product of degradation, a modified aspartyl-adenylate containing an N-acylphosphoramidate linkage, strongly inhibits translation by blocking the function of aspartyl-tRNA synthetase.
Recommended Citation
Metlitskaya, A., Kazakov, T., Kommer, A., Pavlova, O., Prætorius-Ibba, M., Ibba, M. Krasheninnikov, I., Kolb, V., Khmel, I. and Severinov, K. (2006) Aspartyl-tRNA synthetase is the target of peptidenucleotide antibiotic Microcin C. J. Biol. Chem 281, 18033 - 18042. https://doi.org/10.1074/jbc.M513174200
Copyright
American Society for Biochemistry and Molecular Biology
Included in
Amino Acids, Peptides, and Proteins Commons, Biochemistry Commons, Cellular and Molecular Physiology Commons, Molecular Biology Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons, Other Biochemistry, Biophysics, and Structural Biology Commons
Comments
This article was originally published in Journal of Biological Chemistry, volume 281, in 2006. https://doi.org/10.1074/jbc.M513174200