Transfer RNA Recognition by Class I Lysyl-tRNA Synthetase from the Lyme Disease Pathogen Borrelia burgdorferi

Authors

Alexandre Ambrogelly, The Ohio State University
Magali Frugier, The Ohio State University
Michael Ibba, Chapman UniversityFollow
Dieter Söll, Yale University
Richard Giegé, The Ohio State University

Document Type

Article

Publication Date

4-9-2005

Abstract

Borrelia burgdorferi and other spirochetes contain a class I lysyl‐tRNA synthetase (LysRS), in contrast to most eubacteria that have a canonical class II LysRS. We analyzed tRNA^Lys recognition by B. burgdorferi LysRS, using two complementary approaches. First, the nucleotides of B. burgdorferi tRNA^Lys in contact with B. burgdorferi LysRS were determined by enzymatic footprinting experiments. Second, the kinetic parameters for a series of variants of the B. burgdorferi tRNA^Lys were then determined during aminoacylation by B. burgdorferi LysRS. The identity elements were found to be mostly located in the anticodon and in the acceptor stem. Transplantation of the identified identity elements into the Escherichia coli tRNA^Asp scaffold endowed lysylation activity on the resulting chimera, indicating that a functional B. burgdorferi lysine tRNA identity set had been determined.

Comments

This article was originally published in FEBS Letters, volume 579, in 2005. https://doi.org/10.1016/j.febslet.2005.04.001

Recommended Citation

Ambrogelly, A., Frugier, M., Ibba, M., Söll, D. and Giegé, R. (2005) Transfer RNA recognition by class I lysyl-tRNA synthetase from the Lyme disease pathogen Borrelia burgdorferi. FEBS Lett. 579, 2629-2634. https://doi.org/10.1016/j.febslet.2005.04.001

Copyright

Federation of European Biochemical Societies