Suppression of DC-SIGN and gH Reveals Complex, Subset-Specific Mechanisms for KSHV Entry in Primary B Lymphocytes

Authors

Nancy Palmerin, Chapman UniversityFollow
Farizeh Aalam, Chapman UniversityFollow
Romina Nabiee, Chapman UniversityFollow
Murali Muniraju, Beckman Research Institute of City of HopeFollow
Javier Gordon Ogembo, Beckman Research Institute of City of HopeFollow
Jennifer Totonchy, Chapman UniversityFollow

Document Type

Article

Publication Date

7-31-2021

Abstract

Kaposi sarcoma-associated herpesvirus (KSHV) is the causative agent of multiple cancers in immunocompromised patients including two lymphoproliferative disorders associated with KSHV infection of B lymphocytes. Despite many years of research into the pathogenesis of KSHV associated diseases, basic questions related to KSHV molecular virology remain unresolved. One such unresolved question is the cellular receptors and viral glycoproteins needed for KSHV entry into primary B lymphocytes. In this study, we assess the contributions of KSHV glycoprotein H (gH) and the cellular receptor DC-SIGN to KSHV infection in tonsil-derived B lymphocytes. Our results show that (1) neither KSHV-gH nor DC-SIGN are essential for entry into any B cell subset, (2) DC-SIGN does play a role in KSHV entry into tonsil-derived B cells, but in all B cell subtypes alternative entry mechanisms exist, (3) KSHV-gH can participate in KSHV entry into centrocytes via a DC-SIGN independent entry mechanism, and (4) in the absence of KSHV-gH, DC-SIGN is required for KSHV entry into centrocytes. Our results provide a first glimpse into the complexity of KSHV entry in the lymphocyte compartment and highlight that multiple subset-dependent entry mechanisms are employed by KSHV which depend upon multiple cellular receptors and multiple KSHV glycoproteins.

Comments

This article was originally published in Viruses, volume13, in 2021. https://doi.org/10.3390/v13081512

Recommended Citation

Palmerin, N.; Aalam, F.; Nabiee, R.; Muniraju, M.; Ogembo, J.G.; Totonchy, J. Suppression of DC-SIGN and gH Reveals Complex, Subset-Specific Mechanisms for KSHV Entry in Primary B Lymphocytes. Viruses 2021, 13, 1512. https://doi.org/10.3390/v13081512

Copyright

The authors

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.