Inhibition of cPLA2-Mediated Arachidonic Acid Release by Cyclic AMP Defines a Negative Feedback Loop for P2Y-Receptor Activation in Mdck-D1 Cells

Authors

Mingzhao Xing, University of California, San Diego
Steven Post, University of California, San Diego
Rennolds S. Ostrom, Chapman UniversityFollow
Michael Samardzija, University of California, San Diego
Paul A. Insel, University of California, San Diego

Document Type

Article

Publication Date

1999

Abstract

In Madin-Darby canine kidney D1cells extracellular nucleotides activate P2Y receptors that couple to several signal transduction pathways, including stimulation of multiple phospholipases and adenylyl cyclase. For one class of P2Y receptors, P2Y2 receptors, this stimulation of adenylyl cyclase and increase in cAMP occurs via the conversion of phospholipase A2 (PLA2)-generated arachidonic acid (AA) to prostaglandins (e.g. PGE2). These prostaglandins then stimulate adenylyl cyclase activity, presumably via activation of prostanoid receptors. In the current study we show that agents that increase cellular cAMP levels (including PGE2, forskolin, and the β-adrenergic agonist isoproterenol) can inhibit P2Y receptor-promoted AA release. The protein kinase A (PKA) inhibitor H89 blocks this effect, suggesting that this feedback inhibition occurs via activation of PKA. Studies with PGE2indicate that inhibition of AA release is attributable to inhibition of mitogen-activated protein kinase activity and in turn of P2Y receptor stimulated PLA2 activity. Although cAMP/PKA-mediated inhibition occurs for P2Yreceptor-promoted AA release, we did not find such inhibition for epinephrine (α1-adrenergic) or bradykinin-mediated AA release. Taken together, these results indicate that negative feedback regulation via cAMP/PKA-mediated inhibition of mitogen-activated protein kinase occurs for some, but not all, classes of receptors that promote PLA2 activation and AA release. We speculate that receptor-selective feedback inhibition occurs because PLA2activation by different receptors in Madin-Darby canine kidney D1 cells involves the utilization of different signaling components that are differentially sensitive to increases in cAMP or, alternatively, because of compartmentation of signaling components.

Comments

This article was originally published in Journal of Biological Chemistry, volume 274, in 1999. DOI: 10.1074/jbc.274.15.10035

Recommended Citation

Mingzhao Xing, Steven R. Post, Rennolds S Ostrom, Michael Samardzija and Paul A. Insel. Inhibition of cPLA2-mediated arachidonic acid release by cyclic AMP defines a negative feedback loop for P2Y-receptor activation in MDCK-D1 cells. J Biol Chem, 274:10035-10038, 1999.

Copyright

American Society for Biochemistry and Molecular Biology