Document Type
Article
Publication Date
2000
Abstract
The determinants of “basal” activity of signaling pathways regulating cellular responses are poorly defined. One possibility is that cells release factors to establish the set-point of such pathways. Here we show that treatment of Madin-Darby canine kidney cells with the nucleotidase apyrase decreases basal arachidonic acid release and cAMP production 30–40% and that inhibitors of P2Y receptor action also affect basal and forskolin-stimulated cAMP accumulation. Changing medium prominently increases extracellular levels of ATP in Madin-Darby canine kidney, COS-7, and HEK-293 cells. Mechanical stimulation of ATP release likely occurs in virtually every experimental protocol with cultured cells, implicating such release and P2Y receptor activation as critical in establishing the set-point for signal transduction pathways.
Recommended Citation
Rennolds S Ostrom, Caroline Gregorian and Paul A. Insel. Cellular release of and response to ATP as key determinants of the set-point of signal transduction pathways. J Biol Chem, 275 (16):11735-739, 2000.
Copyright
American Society for Biochemistry and Molecular Biology
Included in
Amino Acids, Peptides, and Proteins Commons, Cell Biology Commons, Other Pharmacy and Pharmaceutical Sciences Commons
Comments
This article was originally published in Journal of Biological Chemistry, volume 275, issue 16, in 2000. DOI: 10.1074/jbc.275.16.11735