Protocol for Spatial Profiling of Immune Cells in the Mouse Cornea and Conjunctiva Using NanoString GeoMx DSP and nCounterPro Platforms

Authors

• Lois Kim, Chapman University
• Karthikeyan Ramasamy, Chapman University
• Ajay Sharma, Chapman UniversityFollow

Document Type

Article

Publication Date

3-22-2026

Abstract

Quantification of immune cells and their activation status in a spatially specific manner in a healthy or diseased tissue is a valuable tool in immunology. Here, we present a protocol for the spatial profiling of immune cells in the mouse cornea and conjunctiva. We describe steps for immunostaining with photocleavable oligonucleotide-conjugated antibodies, selecting regions of interest (ROIs), and collecting UV-cleaved oligonucleotides using GeoMx DSP. We then detail procedures for hybridizing collected oligonucleotides, cartridge blotting using nCounter Pro Prep, data acquisition, and data analysis.

Comments

This article was originally published in STAR Protocols, volume 7, issue 2, in 2026. https://doi.org/10.1016/j.xpro.2026.104458

Recommended Citation

Kim, L., Ramasamy, K., and Sharma, A. (2026). Protocol for spatial profiling of immune cells in the mouse cornea and conjunctiva using NanoString GeoMx DSP and nCounterPro platforms. STAR Protoc. 7, 104458. https://doi.org/10.1016/j.xpro.2026.104458

Copyright

The authors

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.