Document Type
Article
Publication Date
6-30-2014
Abstract
Rebamipide is a mucoprotective agent commonly used to prevent nonsteriodal anti-inflammatory drug-induced gastrointenstinal side effects [1]. Human plasma and urine analysis of rebamipide utilizing high performance liquid chromatography (HPLC) have been reported [2]. Recently, we reported on the plasma levels of rebamipide in presense or absence of celecoxib or diclofenac in rats [3] using a modified HPLC method of detection developed by Jeoung et al. [4]. To tailor the method towards use in urinary rebamipide extraction and analysis, the following modifications were made:
- To compensate for high concentrations of rebamipide found in urine, a new rebamipide stock solution was prepared with a final concentration of 50,000 ng/mL.
- Rat urine calibration standards were obtained within the range of 50–1000 ng/mL and 1000–50,000 ng/mL.
- Plasma samples were replaced with urine samples.
Recommended Citation
Dustin L. Cooper and Sam Harirforoosh. 2014. A simple high performance liquid chromatography method for determination of rebamipide in rat urine. MethodsX. 1: 49-55 https://doi.org/10.1016/j.mex.2014.06.002
Copyright
The authors
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
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Animal Experimentation and Research Commons, Medicinal and Pharmaceutical Chemistry Commons, Other Pharmacy and Pharmaceutical Sciences Commons
Comments
This article was originally published in MethodsX, volume 1, in 2014. https://doi.org/10.1016/j.mex.2014.06.002