Effect of Novel Dietary Supplement on Metabolism in vitro and in vivo

Authors

Robert A. Vaughan, Texas Tech University
Ailish C. White, University of New Mexico
Jason R. Beam, University of New Mexico
Nicholas P. Gannon, University of New Mexico
Randi Garcia-Smith, University of New Mexico
Roy M. Salgado, University of New Mexico
Marco Bisoffi, Chapman UniversityFollow
Kristina A. Trujillo, University of New Mexico
Carole A. Conn, University of New Mexico
Christine M. Mermier, University of New Mexico

Document Type

Article

Publication Date

5-5-2015

Abstract

Obesity is an increasingly prevalent and preventable morbidity with multiple behavioral, surgical and pharmacological interventions currently available. Commercial dietary supplements are often advertised to stimulate metabolism and cause rapid weight and/or fat loss, although few well-controlled studies have demonstrated such effects. We describe a commercially available dietary supplement (purportedly containing caffeine, catechins, and other metabolic stimulators) on resting metabolic rate in humans, and on metabolism, mitochondrial content, and related gene expression in vitro. Human males ingested either a placebo or commercially available supplement (RF) in a randomized double-blind placebo-controlled cross-over fashion. Metabolic rate, respiratory exchange ratio, and blood pressure were measured hourly for 3 h post-ingestion. To investigate molecular effects, human rhabdomyosarcoma cells (RD) and mouse myocytes (C2C12) were treated with various doses of RF for various durations. RF enhanced energy expenditure and systolic blood pressure in human males without altering substrate utilization. In myocytes, RF enhanced metabolism, metabolic gene expression, and mitochondrial content suggesting RF may target common energetic pathways which control mitochondrial biogenesis. RF appears to increase metabolism immediately following ingestion, although it is unclear if RF provides benefits beyond those provided by caffeine alone. Additional research is needed to examine safety and efficacy for human weight loss.

Comments

This article was originally published in Journal of Traditional and Complementary Medicine, volume 7, issue 1, in 2015. DOI:10.1016/j.jtcme.2015.03.008

Recommended Citation

Vaughan RA, White AC, Beam JR, et al. Effect of novel dietary supplement on metabolism in vitro and in vivo. J Tradit Complement Med. 2017;7(1):1-8. doi:10.1016/j.jtcme.2015.03.008.

Peer Reviewed

1

Copyright

Center for Food and Biomolecules, National Taiwan University

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.