Distribution of Allatostatin C-like Immunoreactivity in the Central Nervous System of the Copepod Crustacean Calanus finmarchicus

Authors

Caroline H. Wilson, Chapman UniversityFollow
Andrew E. Christie, Mount Desert Island Biological Laboratory

Document Type

Article

Publication Date

3-23-2010

Abstract

The C-type allatostatins (C-ASTs) are a family of highly pleiotropic arthropod neuropeptides. In crustaceans, transcriptomic/mass spectral studies have identified C-ASTs in the nervous systems of many species; the cellular distributions of these peptides remain unknown. Here, the distribution of C-AST was mapped in the nervous system of the copepod Calanus finmarchicus, the major contributor to the North Atlantic’s zooplanktonic biomass; C-AST-immunopositive neurons were identified in the protocerebrum, in several peripheral ganglia associated with feeding appendages, and in the ganglia controlling the swimming legs, with immunopositive axons present throughout the ventral nerve cord. In addition, axons innervating the dorsal longitudinal and ventral longitudinal muscles of the body wall of the metasome were labeled by the C-AST antibody. While the distribution of C-AST-like immunoreactivity was similar between sexes, several differences were noted, i.e., two pair of somata located at the deutocerebral/tritocerebral border in males and immunopositive fibers that surround the genital opening in females. To place the C-AST-like labeling into context with those of several previously mapped peptides, i.e., A-type allatostatin (A-AST) and tachykinin-related peptide (TRP), we conducted double-labeling studies; the C-AST-like immunopositive neurons appear distinct from those expressing either A-AST or TRP (and through extrapolation, pigment dispersing hormone). Collectively, our data represent the first mapping of C-AST in crustacean neural tissue, show that sex-specific differences in the distribution of C-AST exist in the C. finmarchicus CNS, and suggest that the peptide may be involved in the modulation of both feeding and postural control/locomotion.

Comments

NOTICE: this is the author’s version of a work that was accepted for publication in General and Comparative Endocrinology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in General and Comparative Endocrinology, volume 167, issue 2, in 2010. https://doi.org/10.1016/j.ygcen.2010.03.012

The Creative Commons license below applies only to this version of the article.

Recommended Citation

Wilson CH, Christie AE. (2010) Distribution of allatostatin C-like immunoreactivity in the central nervous system of the copepod crustacean Calanus finmarchicus. Gen Comp Endocrin. 1;167(2):252-60. https://doi.org/10.1016/j.ygcen.2010.03.012

Peer Reviewed

1

Copyright

Elsevier

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.