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The objective of this study was to perform a market survey on dry pet foods using enzyme-linked immunosorbent assay (ELISA) to detect total aflatoxins (AFs), ochratoxin A (OTA), and deoxynivalenol (DON). Pet food products (n = 58) marketed for dogs, cats, birds, and rabbits were tested in duplicate with ELISA, and results above the limit of quantitation were confirmed using liquid chromatography tandem mass spectrometry (LC-MS/MS). OTA was detected in one product (rabbit food) and AFs were detected in two products (one dog treat and one bird treat). In contrast, DON was detected in the majority (74%) of products tested. Bird and rabbit products were the most affected by DON, with levels above 0.5 μg/g in 50 and 80% of samples, respectively. One rabbit sample tested positive for both OTA and DON. Overall, the findings of this study revealed a low incidence of AFs and OTA in commercial pet food. Although DON was detected in numerous products, the levels were well below those associated with acute toxic effects.


This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Mycotoxin Research, volume 34, issue 1, in 2017 following peer review. The final publication is available at Springer via DOI: 10.1007/s12550-017-0300-3.

Supplemental Table 1.pdf (37 kB)
Supplemental Table 1

Fig1.pdf (46 kB)
Figure 1

Peer Reviewed



Society for Mycotoxin Research and Springer-Verlag GmbH Germany



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