Simultaneous Bactericidal and Osteogenic Effect of Nanoparticulate Calcium Phosphate Powders Loaded with Clindamycin on Osteoblasts Infected with Staphylococcus Aureus

Authors

Vuk Uskoković, Chapman UniversityFollow
Tejal A. Dasai, University of California - San Francisco

Document Type

Article

Publication Date

2014

Abstract

S aureus internalized by bone cells and shielded from the immune system provides a reservoir of bacteria in recurring osteomyelitis. Its targeting by the antibiotic therapy may thus be more relevant for treating chronic bone infection than eliminating only the pathogens colonizing the bone matrix. Assessed was the combined osteogenic and antibacterial effect of clindamycinloaded calcium phosphate nanoparticles of different monophasic compositions on co-cultures comprising osteoblasts infected with S aureus. Antibiotic-carrying particles were internalized by osteoblasts and minimized the concentration of intracellular bacteria. In vitro treatments of the infected cells, however, could not prevent cell necrosis due to the formation of toxic byproducts of the degradation of the bacterium. Antibiotic-loaded particles had a positive morphological effect on osteoblasts per se, without reducing their viability, alongside stimulating upregulation of expression of different bone growth markers in infected osteoblasts to a higher degree than achieved during the treatment with antibiotic only.

Comments

NOTICE: this is the author’s version of a work that was accepted for publication in Materials Science & Engineering C, Materials for Biological Applications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Science & Engineering C, Materials for Biological Applications, volume 37, in 2014. DOI: 10.1016/j.msec.2014.01.008

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Recommended Citation

Uskoković V, Desai TA. Simultaneous bactericidal and osteogenic effect of nanoparticulate calcium phosphate powders loaded with clindamycin on osteoblasts infected with staphylococcus aureus. Mater Sci Eng C Mater Biol Appl. 2014;37:210-222. doi:10.1016/j.msec.2014.01.008.

Copyright

Elsevier

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.