The primary structure of the hemoglobins from Jaguar (Panthera onco) are presented. Electrophoretic separations without and with a dissociating agent revealed the presence of two hemoglobin components, OL2ß\ and a2 02. The separation of the hemoglobin components was achieved by ion-exchange chromatography. The globin chains were separated by ion-exchange chromatography and also by reversed phase HPLC. The amino-acid sequences of the native chains and peptides were determined by liquid-phase and gas-phase sequencing. N-Acetylserine was detected by FAB-mass spectroscopy as N-terminal group of the ßl chain. The sequences are compared with that of human hemoglobin (Hb A).
Ahmed, A., M. Jahan, Z.H. Zaidi, G. Braunitzer, and R. Goeltenboth. 1987. The primary structure of hemoglobins of the adult jaguar (Panthera onco, Carnivora). Biol. Chem. Hoppe-Seyler 368: 1385-1390. doi: 10.1515/bchm3.1987.368.2.1385