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Osteoporosis, a disease resulting in an increased risk of fracture due to compromised bone, affects 1 in 3 postmenopausal women. Discontinuities in the microarchitecture of bone, such as trabeculae, are seen in postmenopausal osteoporosis. This study aimed to evaluate how sleep deprivation affects the distal femur trabecular thickness of estrogen-deficient rats treated with Zoledronate. 29 ovariectomized Wistar female rats were separated into 4 groups. The control group (C) was housed in standard housing with a 12-hour light/dark cycle and was given an intravenous injection of 0.45 mL of 0.9% saline. The Zoledronate group (Z) were also housed in standard conditions but given an intravenous injection of 50 ug/kg of 10% Zoledronate. The Sleep Deprived group (SD) were given an intravenous saline injection, but were housed in chambers that did not permit sleep for 18 hours, then moved to standard chambers that permitted 6 hours of sleep daily. The Sleep-Deprived Zoledronate group (SDZ) was housed the same as the SD group, but was given an intravenous injection of Zoledronate. After 5 weeks, tibiae and femora were harvested and stored at -80°C until high-resolution micro-CT was done. SDZ had improved distal femur trabecular thickness compared to C (75.5 microns and 67 microns, respectively; p=0.0001). Multi-factor ANOVA revealed a significant interaction between Zoledronate and sleep deprivation (p=0.0078). More research is needed to determine how this interaction impacts executive women who often suffer from sleep deprivation and demanding professions.


This article was originally published in Archives of Epidemiology, volume 4, in 2020.

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