Exploring the Ecological Dynamics of Simple Rhizobial Artificial Populations Using qPCR

Authors

Teresa Hur, Chapman UniversityFollow
Kenjiro Quides, Chapman UniversityFollow
Hagop Atamian, Chapman UniversityFollow

Document Type

Chapman access only poster or presentation

Publication Date

Spring 5-6-2022

Faculty Advisor(s)

Dr. Kenjiro Quides, Dr. Hagop Atamian

Abstract

We explored the dynamics of simple artificial rhizobial populations by conducting a rhizobial passaging experiment and using qPCR. According to the sanctions hypothesis, we expect M. loti genotypes that fix more nitrogen to attain greater population sizes over time. We did not find support for the sanctions hypothesis for our mediocre and non–beneficial combination, but we did find support for the wildtype, mediocre, and non–beneficial strain combination. By utilizing a rapid, relatively cheap, and powerful technique, such as qPCR, future studies will be able to investigate the ecological dynamics of dozens of other M. loti mutants.

Comments

Presented at the Spring 2022 Student Scholar Symposium at Chapman University.

Recommended Citation

Hur, Teresa; Quides, Kenjiro; and Atamian, Hagop, "Exploring the Ecological Dynamics of Simple Rhizobial Artificial Populations Using qPCR" (2022). Student Scholar Symposium Abstracts and Posters. 515.
https://digitalcommons.chapman.edu/cusrd_abstracts/515