Frank Frisch, Oliver Lopez
Osteoporosis is a disease that causes the degradation of bone, leading to an increased risk of fracture. 1 in 3 women over the age of 50 will be affected by Osteoporosis. This study aims to understand how bone is affected by sleep deprivation in estrogen-deficient rats, and how Zoledronate might negate the inimical effects of sleep deprivation on bone. As bone mineral density (BMD) is a crude evaluation of the architectural changes seen in Osteoporosis, trabecular thickness may serve as a better single evaluation of bone health. 31 Wistar female rats were ovariectomized and separated into 4 random groups. The control group(C, n=4) were housed in standard conditions, which permitted a 12 hour light/dark cycle and given a one-time injection of 0.45 mL of 0.9% saline. The Sleep-Deprived group (SD, n=9) received the same injection but were limited to 6 hours of sleep. The Zoledronate group (Z, n=9) were housed in standard conditions but were treated with a one-time injection of 50ug/kg body weight of 10% Zoledronate. The Sleep-Deprived Zoledronate group (SDZ, n=9) were housed in the sleep deprivation conditions and received the same injection as the Z group. After 5 weeks, the rats were sacrificed, and tibiae and femora were collected and stored at -80℃ until a high-resolution micro-CT was done. This communication is a re-evaluation of previously presented data. Multiple comparison tests indicated significant differences between distal femur trabecular thickness of the C and SDZ groups (67.25, 75.5 microns, respectively; p=0.0001). Sleep deprivation improved distal femur trabecular thickness between the Z and SDZ groups (68.375, 75.5 microns, respectively, p=0.00007). Multi-factor analysis of variance (ANOVA) revealed a significant interaction between the treatment and the amount of sleep the rats received (p=0.0078). The increased trabecular thickness found in the sleep-deprived groups may be explained by their additional load-bearing. Our findings encourage consideration for studies of longer duration.
Nolte, Erin, "The Effects of Zoledronate and Sleep Deprivation on the Distal Femur Trabecular Thickness of Ovariectomized Rats: Application of Different Statistical Methods" (2020). Student Scholar Symposium Abstracts and Posters. 392.