Purpose: The purpose of this study was to determine the impact of a 20% and a 40% caloric restrictive diet on bone mineral density (BMD) and bone strength in male rats during the growth period. Methods: 32 male rats were randomly divided into: control groups (C20, n=8 and C40, n=8), a group fed the 20% caloric restrictive diet (D20, n=8), and a group fed the 40% caloric restrictive diet (D40, n=8). An animal in the caloric restrictive diet group was matched and pair fed with an animal in the control group for 6 weeks. Each caloric restrictive diet contained additional vitamins and minerals so that the only variable was a restriction on the amount of calories consumed (i.e. 20% or 40% reduction in caloric intake). Results: There was a 17.5% reduction in body weight for D20 compared to C20. In like manner, there was a 27.5% reduction in body weight for D40 compared to C40. The left tibia BMD for D40 (0.2001± 0.0015 g/cm2) was significantly lower compared to C40 (0.2111 ± 0.0048 g/cm2). Further, the Fmax (the amount of force required to break the tibia expressed in Newtons, N) for D40 (105.73 ± 2.39 N) was significantly lower than C40 (118.73 ± 2.67 N). In contrast, there was no significant difference in tibial BMD or Fmax between D20 and C20. Conclusion: The results suggest that although both caloric restricted groups had a reduction in body weight, only the 40% diet group had a significant decrease in BMD and bone strength.
Lee, Kassandra, "The Impact of a 20% vs. a 40% Caloric Restrictive Diet on Bone Mineral Density During the Growth Period in Male Rats" (2015). Student Research Day Abstracts and Posters. 144.