Reduced Acute Inflammatory Responses to Microgel Conformal Coatings

Authors

Amanda W. Bridges, Petit Institute for Bioengineering and BioscienceFollow
Neetu Singh, Georgia Institute of TechnologyFollow
Kellie L. Burns, Petit Institute for Bioengineering and Bioscience
Julia E. Babensee, Petit Institute for Bioengineering and BioscienceFollow
L. Andrew Lyon, Chapman UniversityFollow
Andrés J. García, Petit Institute for Bioengineering and Bioscience

Document Type

Article

Publication Date

2008

Abstract

Implantation of synthetic materials into the body elicits inflammatory host responses that limit medical device integration and biological performance. This inflammatory cascade involves protein adsorption, leukocyte recruitment and activation, cytokine release, and fibrous encapsulation of the implant. We present a coating strategy based on thin films of poly(N-isopropylacrylamide) hydrogel microparticles (i.e. microgels) cross-linked with poly(ethylene glycol) diacrylate. These particles were grafted onto a clinically relevant polymeric material to generate conformal coatings that significantly reduced in vitro fibrinogen adsorption and primary human monocyte/macrophage adhesion and spreading. These microgel coatings also reduced leukocyte adhesion and expression of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, MCP-1) in response to materials implanted acutely in the murine intraperitoneal space. These microgel coatings can be applied to biomedical implants as a protective coating to attenuate biofouling, leukocyte adhesion and activation, and adverse host responses for biomedical and biotechnological applications.

Comments

NOTICE: this is the author’s version of a work that was accepted for publication in Biomaterials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomaterials, volume 29, issue 35 (2008). DOI: 10.1016/j.biomaterials.2008.08.015

The Creative Commons license below applies only to this version of the article.

Recommended Citation

Bridges, A. W.; Singh, N.; Burns, K. L.; Babensee, J. E.; Lyon, L. A.; Garcia, A. J., Reduced acute inflammatory responses to microgel conformal coatings, Biomaterials 2008, 29 (35), 4605-4615. doi: 10.1016/j.biomaterials.2008.08.015

Copyright

Elsevier

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.