Document Type
Article
Publication Date
8-25-2015
Abstract
There is an increasing demand for rapid detection of waterborne pathogens to monitor drinking water safety. We demonstrate a compact, label-free sensor array for rapid detection of Escherichia coli (E. coli) in contaminated water samples using antimicrobial peptide assisted impedimetric sensor platform. Interdigitated electrode arrays immobilized with the antimicrobial peptide Colicin V (ColV) were used to screen the affinity towards different bacterial strains by monitoring impedance variations in real-time. This ColV assisted impedance biosensor exhibited high selectivity towards Gram-negative strains particularly towards E. coli strains. This selective detection of E. coli from other strains was observed at 102 cfu mL−1, which is clinically relevant. The sensor can detect E. coli from 102 to 106 cfu mL−1 in water sample at pH 7 to 9. These results show that the antimicrobial peptide ColV assisted impedimetric array is capable of rapid, specific detection of E. coli in contaminated water samples.
Recommended Citation
Jiang K, Etayash H, Azmi S, et al. Rapid label-free detection of E. coli using antimicrobial peptide assisted impedance spectroscopy. Anal. Methods. 2015;7(23):9744-9748. doi: 10.1039/C5AY01917F
Copyright
Royal Society of Chemistry
Included in
Bacteria Commons, Environmental Indicators and Impact Assessment Commons, Environmental Monitoring Commons, Fresh Water Studies Commons, Hydrology Commons, Other Environmental Sciences Commons, Water Resource Management Commons
Comments
This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Analytical Methods, volume 7, issue 23, in 2015 following peer review. The definitive publisher-authenticated version is available online at DOI: 10.1039/C5AY01917F.