Selective Binding of AIRAPL Tandem UIMs to Lys48-Linked Tri-Ubiquitin Chains

Authors

Simin Rahighi, Chapman UniversityFollow
Ilauna Braunstein, Technion-Israel Institute of Technology
Nicola Ternette, University of Oxford
Benedikt Kessler, University of Oxford
Masato Kawasaki, High Energy Accelerator Research Organization (KEK)
Ryuichi Kato, High Energy Accelerator Research Organization (KEK)
Tsutomu Matsui, SLAC National Accelerator Laboratory
Ariel Stanhill, Technion-Israel Institute of Technology
Soichi Wakatsuki, High Energy Accelerator Research Organization (KEK)

Document Type

Article

Publication Date

2-11-2016

Abstract

Lys48-linked ubiquitin chains act as the main targeting signals for protein degradation by the proteasome. Here we report selective binding of AIRAPL, a protein that associates with the proteasome upon exposure to arsenite, to Lys48-linked tri-ubiquitin chains. AIRAPL comprises two ubiquitin-interacting motifs in tandem (tUIMs) that are linked through a flexible inter-UIM region. In the complex crystal structure UIM1 binds the proximal ubiquitin, whereas UIM2 (the double-sided UIM) binds non-symmetrically to the middle and distal ubiquitin moieties on either side of the helix. Specificity of AIRAPL for Lys48-linked ubiquitin chains is determined by UIM2, and the flexible inter-UIM linker increases avidity by placing the two UIMs in an orientation that facilitates binding of the third ubiquitin to UIM1. Unlike middle and proximal ubiquitins, distal ubiquitin binds UIM2 through a novel surface, which leaves the Ile44 hydrophobic patch accessible for binding to the proteasomal ubiquitin receptors.

Comments

NOTICE: this is the author’s version of a work that was accepted for publication in Structure. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Structure, volume 24, in 2016. DOI: 10.1016/j.str.2015.12.017

The Creative Commons license below applies only to this version of the article.

Recommended Citation

Simin Rahighi, Ilana Braunstein, Nicola Ternette, Benedikt Kessler, Masato Kawasaki, Ryuichi Kato, Tsutomu Matsui, Thomas M. Weiss, Ariel Stanhill, Soichi Wakatsuki (2016). Selective binding of AIRAPL tandem UIMs to Lys48-linked tri-ubiquitin chains. Structure 24: 412-422. doi: 10.1016/j.str.2015.12.017

Copyright

Elsevier

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.