Altered Self-Assembly and Apatite Binding of Amelogenin Induced by N-terminal Proline Mutation

Authors

Li Zhu, University of California - San Francisco
Vuk Uskoković, Chapman UniversityFollow
Thuan Le, University of California - San Francisco
Pamela DenBesten, University of California - San Francisco
Yulei Huang, Sun Yat-sen University
Stefan Habelitz, University of California - San Francisco
Wu Li, University of California - San Francisco

Document Type

Article

Publication Date

2011

Abstract

Objective—A single Pro-70 to Thr (p.P70T) mutation of amelogenin is known to result in hypomineralized amelogenesis imperfecta (AI). This study aims to test the hypothesis that the given mutation affects the self-assembly of amelogenin molecules and impairs their ability to conduct the growth of apatite crystals.

Design—Recombinant human full-length wild-type (rh174) and p.P70T mutated amelogenins were analyzed using dynamic light scattering (DLS), protein quantification assay and atomic force microscopy (AFM) before and after the binding of amelogenins to hydroxyapatite crystals. The crystal growth modulated by both amelogenins in a dynamic titration system was observed using AFM.

Results—As compared to rh174 amelogenin, p.P70T mutant displayed significantly increased sizes of the assemblies, higher binding affinity to apatite, and decreased crystal height.

Conclusions—Pro-70 plays an important structural role in the biologically relevant amelogenin self-assembly. The disturbed regularity of amelogenin nanospheres by this single mutation resulted in an increased binding to apatite and inhibited crystal growth.

Comments

NOTICE: this is the author’s version of a work that was accepted for publication in Archives of Oral Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Archives of Oral Biology, volume 56, issue 4, in 2011. DOI: 10.1016/j.archoralbio.2010.10.017

The Creative Commons license below applies only to this version of the article.

Recommended Citation

Zhu L, Uskoković V, Le T, et al. Altered self-assembly and apatite binding of amelogenin induced by N-terminal proline mutation. Arch Oral Biol. 2011;56(4):331-336. doi:10.1016/j.archoralbio.2010.10.017.

Copyright

Elsevier

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.