The complete amino-acid sequences of the hemoglobins from the adult Sumatran tiger (Panthera tigris sumatrae) have been determined on automatic liquid- and gas-phase sequenators. The globin chains were isolated by reverse phase HPLC on a column of Nucleosil-C4.7V-Acetylserine was detected by FAB-mass spectroscopy as TV-terminal amino acid residue of the βI chain. Comparing the sequences of the globin chains of the tiger with that of human Hb-A, 23 substitutions were recognized in the a, 29 in βI and 28 in the βII chain.
Jahan, M., A. Ahmed, G. Braunitzer, and R. Goeltenboth. 1989. The amino acid sequence of the adult Sumatran tiger (Panthera tigris, Carnivora) hemoglobins. Biol. Chem. Hoppe-Seylar. 370: 27-33. doi: 10.1515/bchm3.1989.370.1.27