BSA Nanoparticles for siRNA Delivery: Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery

Authors

Haran Yogasundaram, University of Alberta
Markian Stephan Bahniuk, University of Alberta
Harsh-Deep Singh, University of Alberta
Hamidreza Montazeri Aliabadi, Chapman UniversityFollow
Hasan Uludag, University of Alberta
Larry David Unsworth, University of Alberta

Document Type

Article

Publication Date

2012

Abstract

Developing vehicles for the delivery of therapeutic molecules, like siRNA, is an area of active research. Nanoparticles composed of bovine serum albumin, stabilized via the adsorption of poly-L-lysine (PLL), have been shown to be potentially inert drug-delivery vehicles. With the primary goal of reducing nonspecific protein adsorption, the effect of using comb-type structures of poly(ethylene glycol) (1 kDa, PEG) units conjugated to PLL (4.2 and 24 kDa) on BSA-NP properties, apparent siRNA release rate, cell viability, and cell uptake were evaluated. PEGylated PLL coatings resulted in NPs with ζ-potentials close to neutral. Incubation with platelet-poor plasma showed the composition of the adsorbed proteome was similar for all systems. siRNA was effectively encapsulated and released in a sustained manner from all NPs. With 4.2 kDa PLL, cellular uptake was not affected by the presence of PEG, but PEG coating inhibited uptake with 24 kDa PLL NPs. Moreover, 24 kDa PLL systems were cytotoxic and this cytotoxicity was diminished upon PEG incorporation. The overall results identified a BSA-NP coating structure that provided effective siRNA encapsulation while reducing ζ-potential, protein adsorption, and cytotoxicity, necessary attributes for in vivo application of drug-delivery vehicles.

Comments

This article was originally published in International Journal of Biomaterials, volume 2012, in 2012. DOI: 10.1155/2012/584060

Recommended Citation

Yogasundaram, Haran, Markian Stephan Bahniuk, Harsh-Deep Singh, Hamidreza Montezari Aliabadi, Hasan Uludaǧ, and Larry David Unsworth. "BSA Nanoparticles for siRNA Delivery: Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery." International journal of biomaterials 2012 (2012). doi: 10.1155/2012/584060

Copyright

The authors

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