Document Type
Article
Publication Date
2009
Abstract
A specific and sensitive liquid chromatography (LC)-tandem mass spectrometric method for quantitative determination of methylprednisolone (MP) in rat plasma and liver was developed and validated using triamcinolone acetonide as an internal standard. Liquid-liquid extraction using tert-butyl methyl ether was used to extract the drug and the internal standard from plasma and liver. The separation of MP was performed on a C(18) column with a mobile phase of acetonitrile:0.5% formic acid aqueous solution (85:15, v/v) over 4 min. The assay was based on the selected reaction monitoring transitions at m/z 375 -> 161 for MP in plasma, 375 -> 357 for MP in liver, and 435 -> 415 for internal standard in both plasma and liver. The lower limit of quantification was 20 ng/mL based on 100 mu L of plasma or liver homogenate. Intra- and inter-clay assay variations were
Recommended Citation
Zhang, Shuang-Qing, Helen R. Thorsheim, Suman Penugonda, Venkateswaran C. Pillai, Quentin R. Smith, and Reza Mehvar. "Liquid chromatography–tandem mass spectrometry for the determination of methylprednisolone in rat plasma and liver after intravenous administration of its liver-targeted dextran prodrug." Journal of Chromatography B 877.10 (2009): 927-932.
DOI: 10.1016/j.jchromb.2009.02.028
Copyright
Elsevier
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
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Comments
NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography B. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B, volume 877, issue 10, in 2009. DOI: 10.1016/j.jchromb.2009.02.028
The Creative Commons license below applies only to this version of the article.