Date of Award

Spring 5-2022

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Pharmaceutical Sciences

First Advisor

Surya M. Nauli

Second Advisor

Aftab Ahmed

Third Advisor

Ajay Sharma

Fourth Advisor

Amir Ahsan

Abstract

The wavelength is used to identify the exact location and quantify the number of molecules in the spectral imaging system. It aids in identifying materials and studying their quantities by examining how they interact with light. A traditional spectrometer provides spectrum information of chemical compounds, while standard imaging provides the intensity at each pixel of the image. Spectral imaging (SI) combines these two aspects by equipping intensity and spectral data for each pixel. In biomedical research, it is important to assess the inorganics/organics molecules to understand drug activity, cellular toxicity, and distribution. Using the microscopy technique, we found for the first time that spectral imaging can provide a high temporal and spatial resolution of gold (Au) and silver (Ag) nanoparticles (NPs) in fixed, living cells and tissue samples. We discovered that SI has a tremendous potential to study NPs localization and identification in biological samples at a single living cell and tissue, based primarily on the spectra information. In addition, we revealed for the first time that without labeling the drug and destroying the samples, spectral imaging is a novel method that allows us to analyze the dynamics of drug distribution and metabolism in single living cells. The spectral microscopy provides a high image resolution to track and identifies doxorubicin (dox) metabolite, which is doxorubicinol (dox’ol). The microscopy approach confirms that both dox and dox’ol are translocated to the nucleus at different rates, while rhodamine remains in the cytoplasm.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.