A specific and sensitive liquid chromatography (LC)-tandem mass spectrometric method for quantitative determination of methylprednisolone (MP) in rat plasma and liver was developed and validated using triamcinolone acetonide as an internal standard. Liquid-liquid extraction using tert-butyl methyl ether was used to extract the drug and the internal standard from plasma and liver. The separation of MP was performed on a C(18) column with a mobile phase of acetonitrile:0.5% formic acid aqueous solution (85:15, v/v) over 4 min. The assay was based on the selected reaction monitoring transitions at m/z 375 -> 161 for MP in plasma, 375 -> 357 for MP in liver, and 435 -> 415 for internal standard in both plasma and liver. The lower limit of quantification was 20 ng/mL based on 100 mu L of plasma or liver homogenate. Intra- and inter-clay assay variations were
Zhang, Shuang-Qing, Helen R. Thorsheim, Suman Penugonda, Venkateswaran C. Pillai, Quentin R. Smith, and Reza Mehvar. "Liquid chromatography–tandem mass spectrometry for the determination of methylprednisolone in rat plasma and liver after intravenous administration of its liver-targeted dextran prodrug." Journal of Chromatography B 877.10 (2009): 927-932.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Animals Commons, Digestive System Commons, Fluids and Secretions Commons, Hemic and Immune Systems Commons, Medical Biochemistry Commons, Medicinal-Pharmaceutical Chemistry Commons, Pharmacy and Pharmaceutical Sciences Commons