Dr. Aftab Ahmed, Dr. Rukhsana Lalani
Fennel (Foeniculum vulgare) is a biennial Egyptian medicinal plant with an aromatic odor that belongs to the family Apiaceae (Umbelliferae). Fennel seeds are commonly used in traditional medicine, as they are known to have anti-inflammatory, anti-fungal and anti-cancerous activities. The major constituents of the fennel plant are sugars, minerals, essential fatty acids, proteins and fibers. Although, there are numerous studies on the medicinal properties of essential oils of the fennel seeds, but there is limited data reported on the proteins and peptides. The aims of this project are to fully characterize the primary structure of proteins and to determine their biological activities. We present here the preliminary data on the amino acid sequence of Lipid Transfer Protein (LTP) from fennel seeds. The proteins were extracted in Tris/HCl pH 8 buffer and successfully purified by two-dimensional liquid chromatography (2D-LC), using gel filtration chromatography followed by reversed phase HPLC (RP-HPLC). The purity of isolated LTP protein was determined by the SDS gel electrophoresis. The purified protein was loaded on to the PVDF disc and sequenced by automated amino acid sequencer, model PPSQ-31A (Shimadzu). The partial amino acid sequence up to 22 amino acid residues was successfully established. The amino acid sequence similarity was searched using Protein BLAST, which revealed it to be a lipid transfer protein.
Al-Shiyab, Hasan and Aziz, Caroline, "Partial Amino Acid Sequence of Lipid Transfer Protein from Fennel (Foeniculum vulgare) Seeds" (2016). Student Research Day Abstracts and Posters. 209.